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Loss of Rap1 decreased HMVEC proliferation. HMVECs transfected with control, rap1a, rap1b, or rap1a plus rap1b siRNAs were cultured for 72 h in EBM-2 with different concentrations of FGF2 as indicated. Cell proliferation was measured and expressed as the increase in absorbance versus the control. *, P < 0.05 on siRNA control versus that of rap1 siRNA-treated cells (n = 3).
