Skip to main content
. 2008 Jul 14;28(18):5803–5810. doi: 10.1128/MCB.00393-08

FIG. 6.

FIG. 6.

Rap1 depletion abolished Rac activation in HMVECs. (A) HMVECs were starved overnight and stimulated with 25 ng/ml FGF2 for 3 to 60 min. Immunoblotting for Rap1-GTP and total Rap1 levels was performed. (B) Densitometric quantification of protein levels. Bars show means ± standard deviations (SD). *, P < 0.05 (n = 4). (C) HMVECs transfected with control, rap1a, rap1b, or rap1a plus rap1b siRNAs were starved overnight and stimulated with 25 ng/ml FGF2 and 10 μg/ml heparin for 10 min. Immunoblot assays for Rac-GTP and total Rac level were performed. (D) Densitometric quantification of protein levels. Bars show means ± SD; **, P < 0.01; n = 4.