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. 2008 Jul 14;28(18):5803–5810. doi: 10.1128/MCB.00393-08

FIG. 7.

FIG. 7.

Rap1 mediated ERK1/2 and p38 activation by FGF2 in HMVECs. (A) HMVECs were starved overnight and stimulated with 25 ng/ml FGF2 for 3 to 60 min. Immunoblot assays for phosphorylated ERK1/2 and total ERK1/2 are shown. (B) HMVECs transfected with control, rap1a, rap1b, or rap1a plus rap1b siRNAs were starved overnight and incubated with or without 25 ng/ml FGF2 for 10 min. Immunoblot assays for phosphorylated and total ERK1/2 are shown. (C) Densitometric quantification of protein levels. (D) HMVECs were starved overnight and stimulated with 25 ng/ml FGF2 for 3 to 60 min. Immunoblot assays for phosphorylated p38 and total p38 are shown. (E) HMVECs were treated as described for panel B. Immunoblot assays for phosphorylated and total p38 are shown. (F) Densitometric quantification of protein levels. Bars in panels C and F show means ± standard deviations. *, P < 0.05 (n = 3).