FIG. 14.

Effect of Bcl10 and MALT1 knockdown on the inducible association of CARD11 with cofactors in Jurkat T cells. The KD-GFP, KD-Bcl10, and KD-MALT1 cell lines were stimulated with a time course of PMA-ionomycin (P/I) as indicated. Anti-TRAF6 (A), anti-IKKα (B), and anti-caspase-8 (C) immunoprecipitations were performed as described in Materials and Methods. In each panel, Western blotting (WB) with the indicated antibodies (at right) was performed to develop the contents of the immunoprecipitate (top and bottom) and the lysate input (middle). α, anti; IP, immunoprecipitation.