FIG. 2.

Determinants of ID association with the CARD11 ΔID variant. (A) HEK293T cells were transfected with expression vectors for the indicated CARD11 variants and 150 ng of expression vector for either the ID-GST fusion (pEBB-ID-GST) or GST (pEBG). Glutathione-agarose precipitations were performed as described in Materials and Methods. To achieve approximately equivalent expression of each CARD11 variant, the following amounts of each expression vector were used (in ng): wild type (WT), 50; ΔID, 50; ΔIDΔCARD, 1,000; ΔIDΔL1, 150; ΔIDΔCC, 75; ΔIDΔPDZ, 100; ΔIDΔL3, 150; ΔIDΔSH3, 150; ΔIDΔL4, 100; ΔIDΔGUK, 100. Western blotting (WB) with the indicated antibodies was performed to develop the contents of the precipitate (top and bottom panels) and the lysate input (middle panel). α, anti.