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. 2008 Jul 14;28(18):5668–5686. doi: 10.1128/MCB.00418-08

FIG. 9.

FIG. 9.

Determinants of the association of the ΔID with caspase-8 C360S and IKKγ. (A) Anti-FLAG immunoprecipitations were performed as described in the legend of Fig. 8A except that 1,000 ng of the expression vector for FLAG-tagged caspase-8 C360S was used in all indicated samples (+). The following amount (in ng) of expression vector for each CARD11 variant was used: wild type (WT), 100; ΔID, 100; ΔIDΔCARD, 1,000; ΔIDΔL1, 100; ΔIDΔCC, 50; ΔIDΔPDZ, 150; ΔIDΔL3, 125; ΔIDΔSH3, 150; ΔIDΔL4, 50; ΔIDΔGUK, 50. (B) Anti-FLAG immunoprecipitations were performed as described in the legend of Fig. 8A except that 800 ng of the expression vector for FLAG-tagged IKKγ was used in all indicated samples (+). The following amount (in ng) of expression vector for each CARD11 variant was used: WT, 120; ΔID, 120; ΔIDΔCARD, 1,200; ΔIDΔL1, 120; ΔIDΔCC, 120; ΔIDΔPDZ, 180; ΔIDΔL3, 210; ΔIDΔSH3, 180; ΔIDΔL4, 60; ΔIDΔGUK, 60. α, anti; IP, immunoprecipitation; WB, Western blotting.