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. 2008 Jul 21;28(18):5811–5824. doi: 10.1128/MCB.02231-07

FIG. 2.

FIG. 2.

Differential effects of E2 on the CCND1 and PS2 pre-mRNA and mRNA levels. (A) RT-qPCR analysis of E2 effects on the PS2 (black) or CCND1 (white) pre-mRNA levels in nuclear extracts using primers at the 5′ ends of the RNAs at given times (hours) following the onset of E2 treatment. (B) Same experimental conditions as for panel A, but 3′ regions of PS2 and D1 were analyzed. (C) RT-qPCR analysis of E2 effects on the PS2 (black) or CCND1 (white) mRNA levels in nuclear extracts at given times (hours) following the onset of E2 treatment. The amplification of fully spliced mRNAs was done using a forward primer overlapping the exon 1-exon 2 junction and a reverse primer in the last exon. (D) RT-qPCR analysis of effects of E2 on the PS2 (black) or CCND1 (white) mRNA levels in cytoplasmic (C) extracts at given times (hours) following the onset of E2 treatment. Each value was standardized by 18S RNA measurement, and the values at baseline (0 h) for each product were assigned 1. Experiments were performed three times. Data are represented as means ± SEM.