FIG. 10.

Nuclear retention of ribosomal proteins in Dre2-depleted cells. The plasmid pRS316-Rpl25-eGFP was transformed into the Gal-Dre2 strain. Dre2 protein was expressed or depleted by growth in defined medium with raffinose-galactose or raffinose alone, respectively, for 16 h. (A) DAPI and Rpl25-eGFP fluorescent images are shown for the Gal-Dre2-induced (top) and Gal-Dre2-depleted (bottom) cells. (B) Individual cell from Gal-Dre2 culture following Dre2 depletion, showing differential interference contrast (DIC) (a), DAPI (b), and Rpl25-eGFP (c) images. Nuclear accumulation of GFP fluorescent signal appeared only under conditions of Dre2 depletion.