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EPR spectra. EPR spectra for purified Dre2 protein at 45 K and 5 mW (A) and at 6 K and 5 mW (B). The sample (13 mg/ml) in 50 mM Tris-HCl at pH 7.5 containing 50 mM NaCl and 10% glycerol was reduced with 10 mM dithionite. EPR conditions: microwave frequency, 9.45 GHz; modulation amplitude, 8.034 G; modulation frequency, 100 kHz; time constant, 82 ms. Principal g values are indicated.
