FIG. 7.

Increased permeability of K5-expressing E-DMVECs. (A) Schematic drawing portraying ECIS. An 8W10E slide array which was used to monitor real-time attachment and monolayer formation is depicted. The eight culture wells of the slide array are shown, including the eight active electrode areas (circles) and the single counter electrode (rectangle). Front- and side-view enlargements of a single well are shown to highlight the 250-μm-diameter active electrodes at the bottom of each well and the photoresistant layer that insulates the rest of the gold film from the bulk electrolyte. Adapted from Experimental Cell Research (81) with permission of the publisher. (B) ECIS analysis of mock-treated E-DMVECs and E-DMVECs transduced with AdTet alone or together with AdK3 or AdK5 (each treatment or transduction was performed in duplicate, as indicated by the numbers 1 and 2). E-DMVECs were transduced with the indicated Ad constructs for 20 h prior to transfer into an eight-well electrode. (C) At the end of an experiment similar to that described in the legend to panel B, the medium was replaced with fresh medium containing Tet to repress K5 and K3 expression. (D) ECIS upon induction of K5 by Tet removal. The experimental conditions were as described in the legend to panel B, except that the medium contained Tet during plating into the slide array. After 24 h, Tet was removed and resistance was monitored for an additional 65 h.