TABLE 1.
Summary of abundance and DNA-binding activity of ORF50 proteins with deletions and point mutations
| Construct | ORF50 aa | Point mutant(s) | Abundant ORF50 proteina | DNA bindingb | Figure(s) |
|---|---|---|---|---|---|
| F-ORF50/F-691 | 1-691 | No | No | No | 1, 2 |
| F-ORF50/F-691 (KK/EE) | 1-691 | K527E, K528E | Yes | Yes | 1, 2 |
| F-ORF50(KK/AA) | 1-691 | K527A, K528A | Yes | Yes | 1 |
| F-ORF50(KK/RR) | 1-691 | K527R, K528R | Yes | No | 1 |
| F-650 | 1-650 | No | No | No | 2 |
| F-650(KK/EE) | 1-650 | K527E, K528E | Yes | Yes | 2 |
| F-590 | 1-590 | No | Yes* | No | 2 |
| F-590(KK/EE) | 1-590 | K527E, K528E | Yes* | Yes | 2 |
| F-564 | 1-564 | No | Yes* | Trace | 2, 4, 5 |
| F-564(KK/EE) | 1-564 | K527E, K528E | Yes* | Yes | 2, 4, 5 |
| F-490 | 1-490 | No | Yes* | (Yes) | 2 |
| F-D590-650 | 1-589; 651-691 | No | Yes* | No | 2 |
| F-D590-650(KK/EE) | 1-589; 651-691 | K527E, K528E | Yes* | Yes | 2 |
| F-390 | 1-390 | No | Yes* | Yes | 4 |
| F-390+(440-564) | 1-390; 440-564 | No | (Yes*)c | (No) | 4 |
| F-390+(440-564)(KK/EE) | 1-390; 440-564 | K527E, K528E | (Yes*) | (Yes) | 4 |
| F-390+(490-564) | 1-390; 440-564 | No | Yes* | Weak | 4 |
| F-390+(490-564)(KK/EE) | 1-390; 490-564 | K527E, K528E | Yes* | Yes | 4 |
| F-390+(520-564) | 1-390; 520-564 | No | Yes* | Yes | 4 |
| F-390+(520-564)(KK/EE) | 1-390; 520-564 | K527E, K528E | Yes* | Yes | 4 |
| F-390+(535-564) | 1-390; 535-564 | No | Yes* | Yes | 4 |
| F-390+(490-535) | 1-390; 490-535 | No | Yes* | Weak | 4 |
| F-390+(490-535)(KK/EE) | 1-390; 490-535 | K527E, K528E | Yes* | Yes | 4 |
| F-564(KRK/EDE) | 1-564 | K516E, R517D, K518E | Yes* | Yes | 5 |
| F-564(RSK/DSE) | 1-564 | R520D, K522E | Yes* | Yes | 5 |
| F-ORF50(KRK/EDE) | 1-691 | K516E, R517D, K518E | No | Yes | 5 |
| F-ORF50(RSK/DSE) | 1-691 | R520D, K522E | No | Yes | 5 |
| F-564(KK/EE)+IS | 1-564; 490-535 | K527E, K528E | Yes* | No | 6 |
| F-564(KK/EE)+IS(KK/EE) | 1-564; 490-535 | K527E, K528E (2×)d | Yes* | Yes | 6 |
| F-IS + 564(KK/EE) | 490-535; 1-564 | K527E, K528E | Yes* | Weak | 6 |
| F-IS(KK/EE)+564(KK/EE) | 490-535; 1-564 | K527E, K528E (2×) | Yes* | Yes | 6 |
| F-ORF50(KK/EE)+IS | 1-691; 490-435 | K527E, K528E | No | No | 8 |
| F-ORF50(KK/EE)+IS-E | 1-691; 490-435 | K527E, K528E (2×) | Yes | Yes | 8 |
| F-ORF50(KK/EE)+KK9 | 1-691; 525-533 | K527E, K528E | No | Yes | 8 |
| F-ORF50(KK/EE)+EE9 | 1-691; 525-533 | K527E, K528E (2×) | Yes | Yes | 8 |
| F-ORF50(KK/EE)+KKRK | 1-691; 527-530 | K527E, K528E | Yes | Yes | 8 |
| F-ORF50(KK/EE)+EERK | 1-691; 527-530 | K527E, K528E (2×) | Yes | Yes | 8 |
| F-N-IS-ORF50(KK/EE) | 490-535; 1-691 | K527E, K528E | No | Weak | 9 |
| F-N-IS(KK/EE)-ORF50(KK/EE) | 490-535; 1-691 | K527E, K528E (2×) | Yes | Yes | 9 |
| F-PARS | 1-20; 490-691 | No | Yes* | NAe | 10 |
| F-PARS(KK/EE) | 1-20; 490-691 | K527E, K528E | Yes* | NA | 10 |
a
*, We were unable to determine whether the abundant form of ORF50 expressed by truncation mutants was ORF50A or ORF50B.
b
“(Yes)” and “(No)” data for DNA binding are not shown in this study.
c
“(Yes*)” data for protein abundance are not shown in this study.
d
2×, Two occurrences.
e
NA, not applicable.