FIG. 1.

Liver, heart, and skeletal muscle transduction following intraperitoneal injection of AAV8-CMV-lacZ at a dose of 1.0 × 10¹ vg/mouse. (A) Representative photomicrographs of sections of the liver (5 days, 12 days, 6 weeks, and 10 weeks postinjection), heart (10 weeks postinjection), and skeletal muscle (tongue at 10 weeks postinjection). Each section was stained with X-Gal and counterstained with hematoxylin. Scale bars represent 100 μm. (B) AAV8-EF1α-nlslacZ vector map. Locations of restriction enzyme sites and the lacZ probe used for the Southern blot analysis are indicated. (C) Southern blot analysis of AAV8-EF1α-nlslacZ vector genomes in livers 6 weeks postinjection. Ten micrograms of total liver genomic DNA was digested with BglI, BamHI, or KpnI; separated on an 0.8% agarose gel together with undigested sample DNA; transferred onto a nylon membrane; and probed with a lacZ sequence-specific probe. BglI cuts the vector genomes at multiple sites, BamHI cuts near the left terminus of the genome, and KpnI does not cut the vector genome. Head-to-tail molecules, tail-to-tail molecules, supercoiled circular monomer genomes, and large concatemers are indicated with the numbers 1 to 4, respectively. 0.00-1.00, rAAV vector genome copy number standards (ds-vg/dge). Each lane represents individual mouse samples.