FIG. 3.

rAAV vector genome copy numbers in livers before and after a two-thirds partial hepatectomy (PHx) in wild-type (WT) and 44Bri mice. Wild-type and 44Bri mice were injected with 2.0 × 10¹¹ vg of vector AAV8-EF1α-nlslacZ at birth and again injected with 2.5 × 10¹⁰ vg of vector AAV9-LSP-hF.IX at the age of 4 to 7 months. Seventeen days after the rAAV9 vector injection, a subset of the mice underwent a two-thirds partial hepatectomy. All the mice were sacrificed 6 weeks after partial hepatectomy. Vector genome copy numbers of each rAAV8 and rAAV9 vector were determined by Southern blot analysis. Partial hepatectomy (−) samples include liver samples collected at the hepatectomy and those from not-hepatectomized mice. Vertical bars represent standard errors.