FIG. 2.
EIAV SU blocks entry of wild-type strains of EIAV. ED cells (A) or eUVECs (B) were incubated with increasing amounts of 293T cell supernatants that had been either transfected with an empty vector or a codon-optimized EIAVSP19 SU expression vector. The cells were subsequently infected with EIAVMA-1 or EIAVvMA-1c or transduced with VSV-G-pseudotyped EIAV particles that express β-galactosidase. Cells were evaluated for infection or transduction at 40 h. Shown is the ratio of the number of infected or transduced cells in the presence of supernatant divided by the number of infected or transduced cells when no supernatant was added. Data represent the means and standard errors of the means from three separate experiments performed in triplicate. *, P < 0.05; **, P < 0.001.