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Induction of E4orf4 expression in clone 13 cells. E4orf4 expression was induced in clone 13 cells by 1 μg/ml doxycycline. At the indicated times postinduction, cells were either harvested and subjected to Western blot analysis with E4orf4-specific antibodies (A) or fixed and stained with E4orf4-specific antibodies and with DAPI (4′,6′-diamidino-2-phenylindole) (B).
