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NV RNA levels after transfection of pNV-GFP or pNV-GFP alone or with pCI-LC in MVA-T7-infected Vero cells detected by real-time qRT-PCR
| Plasmid | Fold induction in RNA levels (mean ± SD)a |
|---|---|
| pCI | NA |
| pNV-GFP | 1 |
| pNV-GFPΔGDD | 0.92 ± 0.3 |
| pNV-GFP/pCI-LC | 2.1 ± 0.3* |
| pNV-GFPΔGDD/pCI-LC | 1.1 ± 0.2 |
Total RNA was extracted from cell lysates after 20 h of transfection for the real-time qRT-PCR. NA, not available. *, P < 0.05 (compared to the pNV-GFP group).
