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Two regions within the N-terminal half of A3G cooperatively determine the cytoplasmic localization of the full-length protein. Representative images of live HeLa cells transfected with the indicated A3-GFP expression plasmids. Scale bar, 10 μm. Representative cells showing the localization of the indicated full-length A3G single mutants (A) or full-length A3G double mutants (B) are shown.
