FIG. 1.

Schematic representation of the strategy for detection of different HDV RNA species. For detection of nascent antigenomic RNA (AG-RNA, genotype I) and HDAg-encoding mRNA (mRNA-I, genotype I), the in vitro-transcribed genomic RNA (G-RNA) of genotype I was cotransfected with a genotype I/II chimeric HDAg-encoding mRNA (mRNA-I/II) into Huh7 cells. In contrast, for detection of G-RNA produced from cells in which HDV RNA replicated, in vitro-transcribed AG-RNA was used for cotransfection with mRNA-I/II. The origins of each HDV RNA species are indicated.