Skip to main content
. 2008 Jul 23;82(19):9409–9416. doi: 10.1128/JVI.00428-08

FIG. 5.

FIG. 5.

Effects of arginine-13, lysine-72, and serine-177 mutations of S-HDAg on HDV mRNA transcription. (A) The wild-type HDV genomic RNA was transfected together with the S-HDAg-encoding defective mutant (d1nt) or with mRNAs encoding wild-type (WT) S-HDAg or S-HDAg with the R13A, K72A, or S177A mutation into Huh7 cells. (B) Alternatively, the HDV genomic RNA encoding wild-type, R13A, K72A, or S177A HDAg was transfected together with the corresponding S-HDAg mRNA encoding the same mutation. At day 1 posttransfection, total RNAs were extracted, and HDV mRNA was detected by qRT-PCR using the mI-S protocol (Table 2). The data were normalized relative to the values detected in the wild-type HDV RNA transfection. Error bars indicate standard deviations. G + m#, wild-type genomic RNA cotransfected with mutant mRNA; G# + m#, mutant genomic RNA cotransfected with the corresponding mutant mRNA.