FIG. 7.

Effects of curcumin and TSA treatments on HDV genome replication and mRNA transcription. HDV genomic RNA was transfected together with the S-HDAg-encoding mRNA. Four hours after transfection, curcumin (A) or TSA (B) was added at the indicated concentrations and incubated for 24 h. Total RNAs were extracted, and HDV mRNA was detected by qRT-PCR using the mI-S protocol. (C to E) Curcumin (100 μM) was added at 48 h posttransfection for 24 h. (F to H) TSA (25 μM) was added at 4 h posttransfection for 24 h. HDV G-RNA, AG-RNA, and mRNA were detected by qRT-PCR using the G-S, AG-S, and mI-S protocols, respectively. The data were normalized relative to the values without the drug treatment. Error bars indicate standard deviations. (−), negative control (no curcumin or TSA added).