TABLE 1.
Primers used for the HDV RNA species-specific RT-PCR procedurea
| Primer | Positions (5′-3′)b | Sequence (5′-3′)c |
|---|---|---|
| Tagged GS | 416-394 | GCTGTAGCCTACCAGCAGTCAGTCATCACTTTCCTCCTCGCTTCGGTCTC |
| Tagged AGS | 1510-1535 | GTAGGCTACGACTGAAGCCTAGTGCAGTCGAGTTCCTCTAACTTCTTTCTTCC |
| Tagm-T20-F | 938-945 | GCCGCCCTGCAGTTTTTTTTTTTTTTTTTTTTGAGTGGAA |
| mI-R | 1536-1511 | CGGAAGAAAGAAGTTAGAGGAACTCG |
| mII-R | 1376-1361 | GGTCCCGGACGGATCA |
| mI-F | 994-1014 | TCGGCTGGGAAGAGTATATCC |
| TagG-F | GCTGTAGCCTACCAGCAGTCAGTCATC | |
| GS-R | 61-84 | GTCGGTAAAGAGCATTGGAACGTC |
| TagAG-F | GTAGGCTACGACTGAAGCCTAGTGCAG | |
| AGS-R | 166-139 | CCTTCCTAGAGTTTCACTAGCGTTATGG |
a
All of the primers are designed according to the sequence of HDV genotype I, except for primer mII-R, which is designed according to the sequence of HDV genotype II.
b
The positions are presented using the HDV genome numbering of Wang et al. (41). The numbers go “up” and “down” in the 5′-to-3′ direction of genomic and antigenomic HDV RNA, respectively.
c
Underlined sequences are tag sequences that are not present in the HDV genome or antigenome.