Cell killing by the hyperfusogenic P/V-CPI−-G3A mutant. Vero cells were mock infected or infected with the indicated viruses at an MOI of 0.05, 1, or 10. For cells infected with rSV5-TK, medium was used with or without 10 μg/ml GCA as described previously (39). Cell viability was determined at 24, 48, and 72 h p.i. using an MTS viability assay as described in Materials and Methods. Data are from quadruplicate samples and are expressed as a percentage of the value obtained with mock-infected cells, with error bars representing the standard deviation.