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. 2008 Jul 23;82(19):9776–9781. doi: 10.1128/JVI.00917-08

FIG. 5.

FIG. 5.

AME disrupts the ability of Vpu to counter CD317/BST-2/tetherin. 293T cells were cotransfected with a Vpu-defective pNL4-3 derivative (pNL4-3delVpu; delVpu) (13) and a hemagglutinin-tagged CD317/BST-2/tetherin expression vector (tetherin) (15), with or without the Vpu expression plasmid (pCMV-Vphu; Vpu) (16). Transfected cells were treated (+) or not (−) with 10 μM AME and metabolically labeled, and the virus release efficiency was calculated as described in the legend to Fig. 1. Data are means ± SD (n = 3).

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