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. 2008 Jul 30;82(19):9678–9688. doi: 10.1128/JVI.01159-08

FIG. 3.

FIG. 3.

CD4+ T cells with activated phenotype are present in the spinal cord and dorsal root ganglia (DRG) of HSV-1 inoculated mice. Lymphocytes were isolated from iliac lymph node (iLN), spinal cord, and dorsal root ganglia of HSV-1-infected mice on the indicated days (d). Lymphocytes were stained with fluorochrome-labeled antibodies and analyzed by flow cytometry as described in Materials and Methods. The mean number of total CD4+ T cells isolated from iliac lymph nodes ranged from 7.35 × 104 per 106 cells in naïve animals to 1.30 × 105 per 106 cells at the peak on day 8. The number of total CD4+ T cells isolated from pooled spinal cords ranged from 7.63 × 102 per 106 cells in naïve animals to 1.02 × 104 per 106 cells at the peak on day 6. The number of total CD4+ T cells isolated from pooled dorsal root ganglia ranged from 7.12 × 102 per 106 cells in naïve animals to 4.52 × 103 per 106 cells at the peak on day 8.