FIG. 1.

Genome organizations of CSFV Alfort-Jiv and parental non-cp CSFV Alfort-p447. (Top) RNA genome of Alfort-p447 containing one large ORF (box) flanked by 5′ and 3′ NTRs. The positions of the genes encoding the viral structural proteins (capsid protein C and envelope proteins E^rns, E1, and E2) and NS proteins N^pro, p7, NS2-3, NS4A, NS4B, NS5A, and NS5B are indicated. (Bottom) Genome organization of Alfort-Jiv containing an insertion of 1.539 kb (C*-N^pro*) between the N^pro and C genes of Alfort-p447. C*-N^pro* is derived from cp BVDV strain CP8 and encodes a 513-aa fusion peptide encompassing fragments from viral sequences (C*, E^rns*, and N^pro*) and cellular sequences (JivI, JivII, and bovine homologue to human nuclear protein Hcc-1 [Hcc-1*]) (55). The lengths of the bars are not drawn to scale. Positions of primers used for RT-PCR analyses to investigate the genetic stability of Alfort-Jiv are indicated above (Fig. 3B) and below (Fig. 3C) the genomes, respectively. M, silently mutated first codon of C, a genetic marker for Alfort-Jiv. The sizes of Alfort-p447 and Alfort-Jiv are indicated in kilobases (kb) on the right.