Figure 3.

Analysis of ena mutant alleles. Single-embryo Western blot analysis of heterozygous ena mutant stocks. Embryos were collected for 2 h and then aged 5 h. Single embryos were picked and lysed in sample buffer and Western blotted. For the enaGC8 chromosomal inversion, 25% of the embryos were null for Ena protein (top panel, lanes 1, 5, and 9). This blot was stripped and reprobed with an anti-fax antibody to demonstrate the presence of embryonic proteins in all 12 lanes. The ena 23 allele appears to encode an Ena protein with a small truncation, because 25% of the embryos encode only a smaller protein (small arrow) of ∼80 kDa (middle panel, lanes 3, 9, and 12), and 50% of the embryos encode both full-length Ena (large arrow) and the truncated protein. The ena 210 allele appears to encode a full-length protein, because all embryos picked express full-length Ena.