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Sug1 associates with acetylated histone H3 in vivo. HeLa cells transfected with Myc-tagged Sug1 were lysed and subjected to IP with polyclonal antibody against acetylated histone H3 (acH3; lane 3, top). Control samples were subjected to IP with Sug1 antibody (lane 1, top) and isotype control rabbit IgG (rIgG; lane 2, top). IP and lysate control samples (bottom) were subjected to IB for Myc. Results reported are data representative of three experiments.
