FIG. 3.
Point mutations at A/T base pairs are caused by short-tract gene conversion. (A) Rate of nontemplated single-base substitution (PM) compared to that of single-base substitution of Amb origin in wild-type and pseudo-V gene-deleted (ψV−; PCNA+/+/AID−/−/rAID/v-myb/ψV− in Table 1) DT40 cells. Cells were clonally expanded for 2 weeks. Two clones were analyzed from each data set. (B) Nucleotide substitution preferences (as a percentage of the total mutations) deduced from the Vλ sequence analysis of the clones shown in panel A. Preference is shown for mutations categorized as nontemplated base substitution (PM) and those categorized as Amb. Data are from reference 40.