FIG. 2.

DYRK1A interaction with Spry2 is not affected by FGF pathway activation. HEK-293 cells were transfected with expression plasmids encoding FGFR1 and either the wt or the Y55A mutant version of Flag-Spry2, as indicated. At 48 h posttransfection, soluble cell lysates were prepared and subjected to immunoprecipitation with anti-Flag antibody. Both the cell lysates and the immunoprecipitates (IP) were analyzed by Western blotting with the indicated antibodies (PY20, anti-phospo-Tyr antibody; Pi-Erk, anti-phospho-Erk1/2 Thr202/Tyr204).