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. 2008 Aug 4;28(19):6104–6112. doi: 10.1128/MCB.00987-08

FIG. 4.

FIG. 4.

UBC13 function in the DNA damage-signaling pathway is independent of E2 variants MMS2 and UEV1A. (A and C) FK2 and 53BP1 IRIF were not affected in MMS2-deficient cells (A) or by the depletion of MMS2 and UEV1A in HeLa cells (C). (B) Western blot analysis verified siRNA-mediated depletion of MMS2, UEV1A, or both in HeLa cells. CTR, control. (D) Identification of UBC13-associated proteins. A list of proteins that copurified with UBC13 using 293T cells expressing tagged UBC13 is presented. coA, coenzyme A. (E and F) Interaction between UBC13 and its E2 variants is not essential for its function in promoting FK2 and 53BP1 IRIF. The yeast two-hybrid assay verified the interaction or absence of interaction between wild-type or mutant UBC13 with MMS2 or UEV1A (E). The formation of 53BP1 and FK2 IRIF was evaluated in UBC13-deficient cells reconstituted with UBC13 E55Q or F57E mutants (F). (G) Model of UBC13 function in diverse biological processes.