FIG. 5.

PSD1 is required for [rho⁰] signaling to the nucleus. (A) Isogenic [rho⁺] and [rho⁰] lacking the indicated nuclear genes were transformed with low-copy-number plasmids carrying either a PDR5- or PDR15-lacZ fusion gene. Transformants were grown and assayed for β-galactosidase levels as described for Fig. 4. (B) A low-copy-number plasmid containing a PDR3-lacZ fusion gene was introduced into the indicated strains. Transformants were assayed for β-galactosidase activity using the fluorescent substrate as described for Fig. 3. (C) Strains containing the relevant genotypes indicated at the left hand were tested for resistance to cycloheximide (Cyh) using a gradient plate assay as described for Fig. 1. wt, wild type.