TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strain | ||
| E. coli BL21(DE3) | F−ompT gal dcm lon hsdSB (rB− mB−; E. coli B strain), with DE3, a λ prophage carrying the T7 RNA pol gene | |
| Plasmids | ||
| pHUG17.1 | Apr; the 14-kb HindIII fragment from pHPS6 (4) was cloned into pWSK29; contains hugAWXZ, tonB, exbBD, and hugBCD from P. shigelloides | This study |
| pHUG21 | Apr; pHUG17.1 in which the BsiWI fragment containing hugWXZ was removed; contains hugA, tonB, exbBD, and hugBCD from P. shigelloides | This study |
| pHB0.0 | Tcr; contains human α- and β-chain genes controlled by a tac promoter | Somatogen |
| pDSV1 | Tcr; pHB0.0 in which the HindIII-BamHI fragment containing human α- and β-chain genes was removed | This study |
| pHB0.0hug | Tcr; pHB0.0 containing the HindIII fragment from pHUG21; contains human α- and β-chain genes, as well as hugA, tonB, exbBD, and hugBCD from P. shigelloides | This study |
| pWSK29 | Apr; low-copy-number cloning vector | 12 |