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Analysis of hemoglobin production in BL21(DE3) containing various plasmids
| Addition to BL21(DE3) | Plasmid(s) | Absorbance of culture at 24 h at a wavelength of 700 nm | Amt (μg) of soluble hemoglobin per 0.004 OD unit | Amt (μg) of soluble hemoglobin per ml of culture |
|---|---|---|---|---|
| Two-plasmid system | pHB0.0, pHUG21 | 1.18 ± 0.06 | 0.48 ± 0.02 | 142 ± 13.0 |
| One-plasmid system | pHB0.0hug | 1.50 ± 0.08 | 0.65 ± 0.05 | 244 ± 7.4 |
| Hemoglobin genes alone | pHB0.0, pWSK29 | 1.06 ± 0.01 | 0.09 ± 0.01 | 24 ± 2.5 |
