TABLE 1.
Primers used in this study
| Gene | Gene product | Forward primer (5′→3′) | Reverse primer (5′→3′) | Reference |
|---|---|---|---|---|
| adhE | Alcohol-acetaldehyde dehydrogenase | ATC TAT ACT GAT GCA ATG CGT CC | ACG AAC CCA TTG TGG GC | This study |
| aldB | Alpha-acetolactate decarboxylase | AAT GGC AGG CCT TTA CGA G | AGC TTG GTA GGC TTT ACC G | This study |
| als | Alpha-acetolactate synthase | GCA CGT CAT TTC AAA TCA TAC GAA C | CCT CCA TCA CCA GAG TGT GAA TA | This study |
| busAA | Betaine ABC transporter ATP binding protein | CGA TTA AGC AAA CGC AAC AA | AGG TGA GCC CAA AAA TGA AA | This study |
| butB | 2,3-Butanediol dehydrogenase | AGT AGG TGA CCA TGT CGT TG | ACC TCC ACC ATT TCC GC | This study |
| citD | Citrate lyase acyl-carrier protein | GTC TTC AGA TGT GCA AAT CAT G | CGC ATT TTC CAA TGG CAT AAG C | 22 |
| citF | Citrate lyase alpha chain | TCG CTG GAA GAA GTG GTT TT | AGA AGA CGG AGC AAT CCT CA | This study |
| codY | Transcriptional repressor | CAG AAG AAA GCC TTG GCG A | CGT CTT CAC GCC ACA TGA T | This study |
| cspD | Cold shock protein D | TGG CAA ATG GAA CAG TAA AAT G | GCT GAG AAG TGA GCG AAC AA | This study |
| cspE | Cold shock protein E | TAA CAG CTT GAG GTC CAC GA | ACG ACG TTT TCG CTC ACT TC | This study |
| cysS | Cysteinyl-tRNA synthetase | TGG ATG AGC AAG CTG AGT CT | TTC TTT GAC CTC AGG CCC AC | This study |
| cysK | Cysteine synthase | GAG CGT CGT CAG ATT ATT CAA G | GCA TAA ACC AAC CAT TTT CTT C | 22 |
| deoB | Phosphopentomutase | CGA AAG TCC GCT CAA AAC G | TTC CCA GTG CCC AGT CAT T | This study |
| dnaK | Molecular chaperone | GCA GCT CTT GCT TAT GGT CTT G | CGG CAA CCA TCC AGT CAA TG | This study |
| gapB | Glyceraldehyde 3-phosphate dehydrogenase | GTT GTT ATC ACT GCA CCT GG | CAT TGG AGC AAG ACA GTT AGT TG | 22 |
| glnQ | Glutamine ABC transporter ATP-binding protein | AAA GAT GCA ATG CCT GAA ATG | CGT CAA AGA GCA TAA CGT CAG | This study |
| groEL | Chaperonin | TGTA GCT GAT GAT GTT GAT GGA GA | CTT TAC GAC GAT CAC CAA ATC CT | This study |
| gyrA | DNA gyrase subunit A | CAC GGG CAC TTC CTG ATG TA | CCA TAA CTT CAC CGA CAA TA | 22 |
| ldh | l-Lactate dehydrogenase | GTC GCT GTA GCT CTT GCT CG | GTT GAC CAA GGT AGC AGT CG | 22 |
| ldhB | l-Lactate dehydrogenase | AGG TTT TCC ACC TAC CGT CGT | TCT CGA ATT GTC GGA ACT GGT | This study |
| metA | Homoserine O- succinyltransferase | CCT TGA TAA ACC GCA TAA TTC A | GAA GGA CTG AAA CCC AAT CTT C | 19 |
| metB1 | Cystathionine gamma-synthase | GTT GCT AGA GCA AAT TGT CCT G | CAA CGA CCT ATC AAC ATC CAG A | 19 |
| mleS | Malolactic enzyme | ACC ACC CTG AAA ACA TTA CTG AA | CTT GAG TTC CCC AGT CTC CA | This study |
| pfl | Pyruvate-formate lyase | ATG AGC CAC AAG CAT TCT TCT AT | AAG TGG TGA GAC ACA ACA AGA G | This study |
| purM | Phosphoribosylaminoimidazole synthetase | GAT TGC GTA GCC ATG TGC GTC | GCC ACT CCA GCC ACA ACT TG | 22 |
| rpiA | Ribose-5-phosphate isomerase A | TCG AAG AAT TGG GTC GAA GAA | AATTTCATCTGCGCCATCAAC | This study |
| tuf | Elongation factor Tu | CTC TAA AGT ATT GTC TGA CA | GTG TTG ATT GTG ATA CCA CG | 22 |
| 16S rRNA | 16S rRNA | GCT CAC CAA GGC GAT GAT ACA TA | ACC AAC GTT CTT CTC TAC CAA CA | This study |
| 23S rRNA | 23S rRNA | ACA GGA TAG GTA GGA GCC ATT | CGA TTA TGC CAG CGG GTT AG | This study |