TABLE 3.
Comparison between qRT-PCR and microarray data
Data source | Fold change in expressiona
|
||||||
---|---|---|---|---|---|---|---|
cysI | cysDb | sbpb | phoB | oprO | phnCb | algL | |
qRT-PCR | 5.77 | 3.97 | 8.66 | −21.26 | −3.93 | −4.10 | −1.97 |
Microarray | 3.36 | 3.02 | 3.20 | −2.33 | −7.07 | −5.17 | −4.56 |
Relative changes in gene expression of selected up- or downregulated genes in the resistant host, cv. Xanthi, relative to the susceptible host, cv. Samsun.
qRT-PCR results were not statistically different (P ≤ 0.05) between the two cultivars. However, trends in expression patterns were consistent between microarray and qRT-PCR data for these genes.