RISA profiles of microbial communities in soybean stems. (A) Bacterial RISA with primers ITSF and ITSReub. (B) Fungal RISA with primers ITS1F and ITS4. For each treatment, five independent samples were used. Each lane represents an individual plant. Lanes M, size marker (50-1000 MapMarker; Bio Ventures); lanes En1282 and En1314, nonnodulating cultivars En1282 and En1314, respectively; lane Enrei, wild-type nodulating cultivar Enrei; lanes En6500 and Sakukei 4, hypernodulating soybean cultivars En6500 and Sakukei 4, respectively.