. 2008 Jul 25;74(18):5645–5652. doi: 10.1128/AEM.00503-08

TABLE 1.

Primers used in PCR assays to identify stx₁, stx₂, stx_2d, and eae in STEC

Target	Primer	Sequence (5′-3′)	Annealing temp (°C)	Product size (bp)
stx₁	VT1-f^a	TGTAACTGGAAAGGTGGAGTATACA	60	210
	VT1-r^a	GCTATTCTGAGTCAACGAAAAATAAC
stx₂	VT2-f^a	GTTTTTCTTCGGTATCCTATTCC	60	484
	VT2-r^a	GATGCATCTCTGGTCATTGTATTAC
stx₂	Stx2F-21328	TTCTGAGCAATCGGTCACTG	60	779
	Stx2R-22087	CGGCGTCATCGTATACACAG
stx₂	Stx2F-22017	GTCACAGCAGAAGCCTTACG	60	714
	Stx2R-22711	ACCCACATACCACGAATCAG
stx_2d	Stx2d-activatable	CTTTATATACAACGGGTG	54	359
	CKS2	CTGAATTGTGACACAGATTAC
stx_2/2c	Stx2/2c	TTTTATATACAACGGGTA	54	359
eae	EAE-f^a	GACTGTCGATGCATCAGGCAAAG	54	366
	EAE-r^a	TTGGAGTATTAACATTAACCCCAGG
stx₁ inactivation	stx1-KP1	ACAGCTGAAGCTTTACGTTTTCGGCAAATACAGAGGGTGTAGGCTGGAGCTGCTTCG	55	1,600
	stx1-KP2	GTAATTTGCGCACTGAGAAGAAGAGACTGAAGATTCCATCTCCATATGAATATCCTCCTTAG
	stx1-F	TAATCCCACGGACTCTTCC	54
	stx1-R	GCGATGTTACGGTTTGTTACTG
	V-stx1-F	TAATGTGGTTGCGAAGGAAT	54	1,071
	k1	CAGTCATAGCCGAATAGCCT
	k2	CGGTGCCCTGAATGAACTGC	54	928
	V-stx1-R	CGCACTGAGAAGAAGAGACTG
	u49-xbaI	TCATCTAGAGTGGTTGCGAAGGAATTTACC	56	1,168
	L1188-xbaI	TAATCTAGATGAATCCCCCTCCATTATGA
	rpu2	CTGGATGATCTCAGTGGGCGTTCTTATGT	58	4,159
	rpl4132	AGACTGCGTCAGTGAGGTTCCACTATGC
	pDS132-F	CCTGTTTGAAGATGGCAAG	50	1,708
	pDS132-R	GGATGTAACGCACTGAGAAG

Data from reference 20.