FIG. 1.
Effect of lactic acid on biomass formation in glucose-limited anaerobic chemostat cultures of S. cerevisiae CEN.PK 113-7D. Each data point represents an independent chemostat culture which was grown to steady state at a dilution rate of 0.10 h−1. Based on the data obtained at pH 5.0 (•), the Henderson-Hasselbach equation was used to estimate an equivalent stress at pH 3.5 (dashed line), assuming that the undissociated acid was solely responsible for the observed decrease of the biomass concentration. Experimental data obtained at pH 3.5 (○) did not correlate with this prediction.