TABLE 3.

Checklist of elements of collection and preservation criteria

Element(s) of collection and preservation criteria
Target, namely, the microorganism, toxin, or analyte (e.g., such as DNA, RNA, protein, and elements)
Sample integrity and the maintenance of its true state or condition, such as viability
Sample stability and the maintenance of its true state or condition over time, particularly during transport or storage
Recovery, specifically, the efficiency of collection/recovery of the target from the substrate or from surfaces (especially when the entire specimen is not collected and sent to the laboratory)
An understanding of the influence of the sample matrix and conditions, for example, wet samples, dry samples, or frozen samples, and unwanted materials that may be collected along with the target
Compatibility of the collection device or material, whether the material is inert or interacts with the target, with subsequent signature extraction and analysis methods; a description of any special procedures needed for the efficient recovery of the target from the collection device
Packaging and storage strategies that maintain the integrity and stability of the target and comply with applicable safety and transportation regulations
Personnel requirements, namely, the roles of personnel (e.g., in a 2-person method) and their training, proficiency, and safety