TABLE 1.
Diagnostic primers used in this study for screening eubacteria (excluding the primary symbionts Carsonella spp.) and symbionts of Bactericera (Paratrioza) cockerelli (Sulc)
| Target DNA | Target | Primer name | Primer sequence (5′-3′) | Product size (bp) | Reference |
|---|---|---|---|---|---|
| All eubacteria | 16S-23S rRNAs | 10F | AGTTTGATCATGGCTCAGATTG | ∼2,600 | 24 |
| 480R | CACGGTACTGGTTCACTATCGGTC | 24 | |||
| “Candidatus Liberibacter psyllaurous” | 16S rRNA | Lp Frag 1-(25F) | CTGATCATGGCTCAGAACGA | 400 | This study |
| Lp Frag 1-(427R) | CGGCGAAAGAGCTTTACAAC | This study | |||
| 16S rRNA | Lp Frag 2-(266F) | AGGCCTACCAAGGCTACGAT | 872 | This study | |
| Lp Frag 2-(1138R) | CACCTTCCTCCGGATTATCA | This study | |||
| 16S rRNA-ISR | Lp Frag 3-(1024F) | CAGCTCGTGTCGTGAGATGT | 662 | This study | |
| Lp Frag 3-(1686R) | TTCGAACTACCGACCTCACC | This study | |||
| 16S rRNA-ISR-partial | Lp Frag 4-(1611F) | GGTTGATGGGGTCATTTGAG | 918 | This study | |
| 23S rRNA | LP Frag 4-480R | CACGGTACTGGTTCACTATCGGTC | This study | ||
| Bactericera cockerelli | 28S D2 rRNA | D2 BC F | GCGAGGACTCAGTTTCGTGT | 175 | This study |
| D2 BC R | AGAGCTCGACTCGGATTGTC | This study |