Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
TLC analysis of soluble products in the reaction mixtures of PcCel45A with various glycans. PcCel45 (1.0 μM) was incubated with 0.5% substrate in 50 mM sodium acetate buffer (pH 5.0) at 30°C for 1 h. The products were applied, developed, and detected as described in Materials and Methods. Lanes 1, MCC; 2, PASC; 3, CMC; 4, lichenan; 5, barley β-glucan; 6, glucomannan; 7, xylan. In each lane, reaction mixtures with (+) and without (−) enzyme were applied.
