TABLE 1.
Substrate | Activity (min−1) |
---|---|
MCC | ND |
PASC | 5.4 |
CMC | 6.4 |
Lichenan | 19 |
β-Glucan from barley | 13 |
Glucomannan | 1.3 |
Xylan | ND |
PcCel45 (1.0 μM) was incubated with 0.5% substrate in 50 mM sodium acetate buffer (pH 5.0) at 30°C for 1 h. The formation of reducing sugar was quantified by the p-hydroxybenzoic acid hydrazide method as described in Materials and Methods. ND, not detected after 120 h of incubation.