TABLE 1.

Primers used in the study

Locus	Primers	Sequence (5′-3′)	Tma (°C)	PCR product size (bp)
IGSNOD	nod283R_HEX	GCGATCCATTCACGCTGAT	55	304
	nod717F_FAM	CAGTTCTGGCATTCAAGC
typA	typ1706R_HEX	GGGCTGGTAGTCGTGGAACA	59	258
	typ1707F_FAM	GCACAAGGATGAGAACGGACAG
virB11 and avhB11	virB1478F_FAM	SGGTWSSGGCAAGACGAC	56	212
	virB1479R_HEX	AGCARWATTCGGTCCGGC
virB11	virB11F_383	GAGCGGCTACAGATCTGTT	53b	492
	virB11R_860	CRTCGCGYATCTCGCCGAGC
avhB11	avhB11F_383	GARCGVATCCAGATCGTCA	53b	483
	avhB11R_860	CRTCKCGMARYTCCTGMAGC
16S rRNA gene	FGPS 1509	AAGGAGGGGATCCAGCCGCA	57	1,500
	FGPS 6	GGAGAGTTAGATCTTGGCTCAG

^aT_m, melting temperature.

^bA touchdown PCR program was used (20 cycles of annealing at 63°C [decreasing by 0.5°C at each cycle] followed by 25 cycles of annealing at 53°C).