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Spnoc3+is an essential gene. (A) Wild-type cells (control) and cells expressing SpNoc3-HA in the Spnoc3Δ:ura4+ disruption haploid strain (SpNoc3 shutoff) were examined for growth on plates in the presence or absence of thiamine. (B) Wild-type Spnoc3+ (CHY154) and Spnoc3ts mutant (CHY222) strains were examined for growth on plates at 25°C and 36°C. (C) Microscopic analysis of the Spnoc3ts mutant strain grown on plates for 72 h at 25°C and 36°C.
