FIG. 3.

SpNoc3 is required for normal cell division. (A) The DNA contents of the wild-type Spnoc3⁺ strain and the Spnoc3^ts mutant strain grown for 20 h at the permissive (25°C) and restrictive (36°C) temperatures were determined by flow cytometric analysis. (B) Microscopic examination of Spnoc3^ts mutant cells grown in liquid medium at 36°C for 20 h. (C) Flow cytometric analysis of the DNA content of wild-type Spnoc3⁺ (CHY154) and Spnoc3^ts mutant (CHY222) cells grown in liquid media at 36°C for 4 h and then incubated in the absence (−HU) or presence (+HU) of HU for an additional 4 h at 36°C. (D) Microscopic examination of wild-type Spnoc3⁺ and Spnoc3^ts mutant cells grown in liquid medium at 36°C for 4 h and then incubated in the presence of HU for an additional 4 h at 36°C.