FIG. 1.

Nuclear positioning within C. albicans mating projections. Mating projections were induced in opaque a cells with alpha pheromone. The HTB-GFP-tagged wild-type (WT; RBY1009) or Δkar3/Δkar3 (RSY19) strain or a Δdyn1/Δdyn1 (RSY136) strain expressing HHF1-GFP was incubated in Spider medium containing alpha pheromone for 18 h at 25°C. Representative live cells were photographed, and fluorescent nuclear images were superimposed over DIC images. Cells from each strain produced mating projections containing nuclei that migrated close to the tip of the mating projection (A and B, C and D, and E and F, respectively). Bars, 5.0 μm.