FIG. 8.

Comparison of spindle morphologies of C. albicans wild-type and Δkar3/Δkar3 cells. Htb-RFP- and Tub2-GFP-tagged wild-type (WT; RSY150) and Δkar3/Δkar3 (RSY155) strain cells in logarithmic phase were imaged by live fluorescence microscopy. The cells in rows A, B, D, and F were grown in YPD at 30°C, and the cells in row C were cultured in YPD medium plus 10% serum at 37°C. Wild-type cells contained mitotic spindles in cells where the DNA was stretched between mother and daughter cells (A to C). In contrast, normal mitotic spindles were absent in Δkar3/Δkar3 cells (Tub2-GFP pattern in panels D to F), even in cells that were undergoing anaphase (Htb-RFP pattern). Despite the absence of normal mitotic spindles in kar3 mutants, cells still underwent nuclear division, evidenced by DNA spanning between mother and daughter cells (D to F). Bar, 5.0 μm.