TABLE 3.
MTLa × MTL α | % Tetraploid | % Diploid |
---|---|---|
Wild type a × wild type α | 63 | 37 |
Δkar3/Δkar3a × Δkar3/Δkar3 α | 0 | 100 |
Δkar3/Δkar3+KAR3a × Δkar3/Δkar3 α | 33 | 67 |
Shown are the percentages of tetraploid cells formed following mating crosses between the opaque wild-type (RBY1132 and RBY1132), Δkar3/Δkar3 (RSY12 and RSY11), and Δkar3/Δkar3 strains with the Δkar3/Δkar3+KAR3 (RSY12 and RSY26) strains. Mating zygotes were picked by micromanipulation and analyzed by PCR of the MTL locus as described in Materials and Methods.