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. 2008 Jun 27;7(9):1573–1581. doi: 10.1128/EC.00427-07

FIG. 2.

FIG. 2.

Analysis of mus-51::bar, ▵hsf1 transformants. (A) Schematic diagram showing the location of SacI restriction enzyme sites in the region of hsf1. (Left) Wild-type hsf1 locus; (right) locus with the hsf1 gene deleted and replaced with hph. (B) Southern blot of mus-51::bar, ▵hsf1 strains. Genomic DNA digested with SacI and probed with DIG-labeled DNA homologous to a region 5′ of the hsf1 ORF. Lanes: 1, 7, and 12, DNA ladder; 8, control parental mus-51 genomic DNA; 2 to 6, 9 to 11, 13, and 14, DNA from different transformants.