FIG. 4.
Response of wild-type and aconidial strains to heat shock. Strains inoculated onto race tubes were germinated and grown in LL at 25°C (A and B) or 34°C (C) for 48 h before transfer to DD at 25°C (A and B) or 34°C (C). After 24 h in DD, cultures were exposed to a 1-h 50°C heat shock and then transferred back to DD at 25°C (A and B) or 34°C (C). Growth was monitored twice daily over the following 9 days. The graph shows the percentages of recovery for the following strains against time (h) after heat shock. (A) 30-7 bd (n = 21), 16-31 bd (n = 18), 16-21 hsf2KO,bd (n = 17), and 16-6 hsf2KO,bd (n = 18). Numbers preceding the strain names refer to the cross and progeny identifiers, e.g., 16-31 indicates cross number 16 and progeny number 31. (B) 16-21 hsf2KO,bd (n = 17), 16-6hsf2KO,bd (n = 18), acon-2,bd (n = 17), and fl,bd (n = 21). (C) 16-31 bd (n = 17), 16-21hsf2KO,bd (n = 18), fl,bd (n = 15), and acon-2,bd (n = 18). The average results of three independent experiments are plotted. HS, heat shock.